The function of the nucleus of our cells is to coordinate the essential processes of DNA storage, maintenance, replication and translation. All of these different processes require a different set of proteins. In my research, I am interested in how the different proteins work together and are regulated in space and time in order to faithfully fulfil the nucleus function. I am employing single-molecule quantitative fluorescence microscopy and super-resolution microscopy on eukaryotic cells to obtain spatiotemporal information of nuclear proteins during the cell cycle. This high -resolution view of nuclear protein localization ties into our understanding of genomic chromatin architecture and should provide a better understanding of gene maintenance and regulation.